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Internal curing (IC) uniformly provides additional water required for extended hydration of cementitious materials. The aim of this study is to investigate the effects of substitution lightweight fine aggregate (LWFA) as an IC water carrier on the rate of hydration reactions, capillary pore water potential development, moisture content, and transport properties of cement-based materials. For this purpose, five mortar and 14 concrete mixtures with different W/C (0.35, 0.42 and 0.5), supplementary cementitious materials (20% Fly Ash and 7.5% silica fume substitution) and LWFA substitutions (10, 20, 30 and 40%) are investigated. The results show that IC improves cementitious materials hydration and postpones capillary pore water potential development. Also, customized relationships between the electrical properties (permittivity and resistivity) and moisture content of the mixtures are developed. In addition to compressive strength, the effect of IC on surface electrical resistivity and rapid chloride migration of the mixtures are investigated. Due to the effect of IC water carriers on the material properties, there is an optimum LWFA substitution considering mechanical properties while transport properties are improved on all replacement percentages.